RICERCA FINALIZZATA

Background:
Glioblastoma (GBM) is one of the major lethal neoplasm in human, with a median survival time of less than a year. Glioma that originate from glial tissue account for about 60% of primary tumors in the central nervous system in adults. Although substantial progress has been made in the molecular basis of GBM, in general, this tumor is still incurable. Genetic alterations occurred during GBMs progression involve the pathways that regulate cell growth and cell cycle. At molecular level, GBMs are characterized by a wide array of genetic and epigenetic alterations as inactivation of tumor suppressor genes (p53), constitutive activation of membrane receptors (EGFR), induction of prosurvival pathways (PI3K/AKB), and metabolic dysfunctions. The ubiquitin-proteasome system is emerging as an important control mechanism of cell growth, survival and metabolism. Dysregulation of the UPS (ubiquitin-proteasome system) has been implicated in a wide array of human pathologies, including cancer (4, 5). Ubiquitin-proteasome pathway, in fact, is the principal mechanism to eliminate unneeded or damaged proteins (6). Removal of tumor suppressors or pro-apoptotic factors could, thus, play an important role in glioma malignancy. praja2 belongs to a growing family of widely expressed mammalian RING proteins with intrinsic ubiquitin ligase activity. praja2 is expressed in most of the neurons from different brain regions and in glial cells, and plays a major role in compartmentlised cAMP signaling. We found that praja2 levels are markedly upregulated in high-grade human glioma and its expression levels positively correlated with the grading and invasiveness potential of the biopsied tumors. Genetic knock-down of praja2 severely inhibited proliferation of human glioblastoma (GBM) cells in vitro and severely affected xenograft GBM growth in vivo. At mechanistic level, we found that praja2 binds and degrades MOB1, a component of the NDR/LATS kineses and positive regulator of the tumor suppressor Hippo pathway. The identification of MOB1 as interactor and substrate of praja2 functionally links the UPS to the deregulated Hippo pathway in tumor cells. praja2-directed degradation of MOB1 by the ubiquitin-proteasome system (UPS) may inhibits the Hippo pathway, promoting cell proliferation and GBM growth.

Aims:
PROBING PRAJA2 FUNCTION IN GBMs. Preliminary data indicate that praja2 levels are upregulated in human glioma. We wish to extend this analysis and invstigate praja2 expression in a larger number of glioblastoma samples. Furthermore we wish to selectively interfere with praja2 expression and evaluate the biological consequences of such genetic manipulation in glioma growth in vivo.

Study Design:
Effects of gene silencing praja 2 on proliferation of human glioblastoma cells in vitro and in vivo.

Investigators
Dr. Antonietta Arcella Researcher Molecular Biologist  IRCCS Neuromed
Dr. M. Antonietta Oliva   IRCCS Neuromed
Prof. Antonio Feliciello  Università Federico II  Napoli
Dr Luca Lignitto Resercher  Università Federico II  Napoli
Dr.ssa Maria Sepe Resercher  Università Federico II  Napoli

Summary Preliminary Data:
1.praja2 expression in human glioma. We investigated the expression profile of praja2 in human glioma tissues, comparing tumors with different recurrence rate and metastatic potential. Tissues samples were derived from first biopsies of patients that underwent to brain surgery for low-grade astrocytoma (grade I and grade II) or high-grade glioma (glioma III and GBM). Tissue samples were homogenized, the lysates were size-fractionated on SDS-PAGE and immunoblotted with anti-praja2 antibody.Figure 1A shows that low levels of praja2 were visible in lysates from control and astocytoma (grade II), whereas the amount of praja2 increased by two-three folds in lysates from high grade (grade III and GBM) gliomas. Quantitative analysis is presented in figure 1B.

2. praja2 is required for tumor growth in vivo Figure 2 A. U87MG cells were transfected with siRNAc or siRNApraja2 duplexes. Twenty-four hours from transfection, cells were harvested and lysed. Total lysates were immunoblotted with anti-praja2 and anti-actin (loading control). A representative autoradiogram is shown. Cumulative data of praja2 levels in siRNAs-transfected cells is shown (right panel). antibody Figure 2  B. U87MG cells transiently transfected with siRNAs

Materials and Methods:were subcutaneously implanted into CD1 nude mice. Four weeks later, mice were sacrificed, the tumors exiced and weighted. Cumulative data of tumor weight are expressed as mean ± S.E. (lower panel).

Task A. praja2 is widely expressed in mammalian tissues, including neurons and glial cells of most regions of the mammalian brain where it has been implicated in critical aspects of cAMP signaling and gene transcription (9). Our preliminary data indicate that praja2 is markedly upregulated in high grade gliomas (see “Preliminary data” Fig. 1). Therefore, we wish to extend the expression analysis of praja2 in a wide number of glioma samples, comparing tumor lesions with different recurrence rate and metastatic potential. Tissues samples derived from first biopsies of patients that underwent to brain surgery for low-grade astrocytoma (grade I and grade II) or high-grade glioma (glioma III and GBMs) are already available at Neuromed Institute. The samples will be analyzed by immunoblot analysis using two distinct specific antibodies directed against praja2 (9). The malignant potential of the tumors will be assessed monitoring the levels of proliferative markers (PCNA and Ki-67).

Task B. The pathogenic role of praja2 in glial tumors will be confirmed by knocking-down endogenous praja2 in GBM cells and monitoring the effects of such manipulation on tumor cell growth in vitro and in vivo. Primary cultures of GBM cells or tumor stem cells will be transiently transfected with siRNA duplexes targeting endogenous praja2 (siRNApraja2) or with control non-targeting siRNAs (siRNAc). Immunoblot analysis on an aliquot of transfected cells will confirm the efficacy of siRNApraja2 in downregulating endogenous praja2, as previously assessed (9). For in vitro studies on GBM cells, we will monitor the following parameters: 1) activation of ERK1/2 and ERK-dependent gene transcription; For in vivo analysis, siRNA-transfected cells will be subcutaneously injected into nude mice. Preliminary data strongly suggest that praja2 is required for GBM growth in vivo. Orthotopic GBM models will be also used. Tumor volume will be monitored at different weeks post-implantation by Real-Time-Bioluminiscence Image, PET-TC and immunohistochemistry. Ki67 and PCNA will be used as proliferative markers of the implanted tumors.

Main Expected Results and Impact:
The goal of this project is to understand the role of praja2-MOB1 pathway in glioblastoma development and to determine if and how these proteins affect glioma cancer progression. These findings may identify praja2 as a novel cancer-associated gene whose expression marks aggressiveness potential of human glioma. Thus, praja2 may serve as novel biomarker for tumor aggressiveness and novel target for GBM therapy.

Significance and Relevance for National Health System (SSN):
Blocking gene Praja2 by Si-RNA, may represent a new therapeutic strategy in the future for the treatment of brain tumors

RICERCA CORRENTE

Stratificazione prognostica molecolare dei gliomi ad alto grado nei bambini: studio della via transcrizionale Wnt/b-catenin nei pHGG

I tumori cerebrali sono oggi la principale causa di morte per cancro nei bambini. I gliomi pediatrici ad alto grado  (classificati dall’Organizzazione Mondiale per la Sanità (OMS) come astrocitomi anaplastici grado III e glioblastomi grado IV) rappresentanoil15% di tutti i tumori cerebrali ed hanno una sopravvivenza a 3 anni di meno del 20% con un’elevatà morbilità.

Tali neoplasie sono istologicamente  indistinguibili da quelle dell’adulto, tuttavia mentre per i gliomi maligni dell’adulto, negli ultimi anni, si sono accumulate una notevole massa di informazioni sugli eventi molecolari alla base della progressione neoplastica, poco si conosce sui meccanismi alla base delle forme pediatriche. Recenti studi di analisi di espressione genica hanno permesso di identificare nei glioblastomi dell’adulto eventi molecolari che permettono di stratificarli in categorie prognosticamente significative. Questo contrasta con gli scarsi dati che abbiamo sui meccanismi molecolari alla base dello sviluppo dei glioblastomi pediatrici; ciò è in gran parte dovuto sia alla mancanza di materiale biologico congelato sia alla dispersione del materiale istologico. Sebbene nella maggioranza dei casi essi insorgano come “gbm de novo”, tuttavia, a differenza di quelli dell’adulto, hanno mutazioni della p53 e solo raramente amplificazioni dell’EGFR. Inoltre, occasionali studi di citogenetica hanno mostrato che i gliomi maligni pediatrici hanno anomalie cromosomiche differenti da quelli dell’adulto. Dato che la maggioranza degli attuali trattamenti sono basati sull’estrapolazione delle conoscenze molecolari delle neoplasie dell’adulto, è necessaria una maggiore comprensione della patogenesi molecolare dei gbm pediatrici al fine di sviluppare terapie  più efficaci. Uno studio recente  (Faury et al. J Clin Oncol 25:1196-1208, 2007-11-27)  ha mostratoilruolo chiave dell’attivazione combinata delle vie di Ras e Akt nel controllo della crescita e nella sopravvivenza nei gliomi maligni dell’adulto. Al contrario, sempre in questo studio. l’attivazione della via di  Notch identificava una popolazione di pazienti con prognosi meno severa. Questo studio ha ovvie implicazioni per lo sviluppo di terapie efficaci per i gliomi maligni in quantoiltrattamento ottimale di tali neoplasie non può che avvenire che agendo su specifici target  molecolari. Nel campo dei gliomi maligni pediatrici uno studio di profilo trascrizionale suggerisce due sottotipi di gbm  uno associato all’attivazione di Ras/Akt ed un andamento clinico più aggressivo ed un altro, privo di attivazione di Ras/Akt, con prognosi meno grave. Tuttavia a differenza dei gbm dell’adulto,ilsottotipo di gbm pediatrico a prognosi più favorevole non è associato con l’attivazione di Notch e non presenta marker proneuronali.

Sulla base di quanto sopradetto, è evidente che i gliomi maligni dell’infanzia sebbene istologicamente simili a quelli dell’adulto hanno meccanismi molecolari di progressione neoplastica differenti da quest’ultimi. L’accurata caratterizzazione di tali meccanismi identificherebbe categorie prognostiche di bambini affetti da questa grave patologia permettendo di modulare una corretta terapia evitando sovra o sottotrattamenti. Inoltre tale caratterizzazione permette di sviluppare terapie innovative mirate su specifici target molecolari.

Nel corso del 2012 studieremoil pathway Wnt/beta-catenina nei gliomi pediatrici.

Il pathway Wnt/beta-catenina oltre ad essere implicato nello sviluppo embrionale e nella sopravvivenza di cellule staminali adulte, è coinvolto nella patogenesi di numerosi tumori e la positività nucleare di beta-catenina è indicativa di attivazione della via transcrizionale .. Nei gliomi sono stati riportati pochi casi di mutazioni nel gene di beta catenina, ctnnb1, mentre sono coinvolte, nell’attivazione di Wnt, mutazioni dei componenti a monte di beta-catenina o regolazioni epigenetiche, come l’ipermetilazione dei promotori di inibitori di Wnt nei gliomi astrocitari maligni dell’adulto. E’ stato dimostrato in linee di astrocitoma maligno che l’inibizione di Wnt tramite alfa2-macroglobulina  determina una riduzione  della proliferazione e della malignità cellulare. Questo contrasta con quanto si osserva nel medulloblastoma  in cui  l’attivazione di Wnt  dimostrata dall’accumulo nucleare di beta-catenina si associa ad una prognosi migliore . A tutt’oggi mancano informazioni  sull’ attivazione di Wnt nei pHGG.

-    Antonelli M, Massimino M, Morra I, Garrè ML, Gardiman MP, Buttarelli FR Arcella A, Giangaspero F. Expression of pERK and pAKT in pediatric high grade astrocytomas: Correlation with YKL40 and prognostic significance. Neuropathology. 2011 Oct 6. doi: 10.1111/j.1440-1789.2011.01252.x. I.F. 1.605
-    Massimino M, Solero CL, Garrè ML, Biassoni V, Cama A, Genitori L, Di Rocco C,  Sardi I, Viscardi E, Modena P, Potepan P, Barra S, Scarzello G, Galassi E, Giangaspero F, Antonelli M, Gandola L. Second-look surgery for ependymoma: the Italian experience. J Neurosurg Pediatr. 2011 Sep;8(3):246-50. I.F. 1.098
-    Frati A, Pichierri A, Bastianello S, Raco A, Santoro A, Esposito V, Giangaspero F, Salvati M. Frameless stereotactic cerebral biopsy: our experience  in 296 cases. Stereotact Funct Neurosurg. 2011;89(4):234-45. I.F. 1.882
-    Hasselblatt M, Gesk S, Oyen F, Rossi S, Viscardi E, Giangaspero F, Giannini C, Judkins AR, Frùhwald MC, Obser T, Schneppenheim R, Siebert R, Paulus W. Nonsense  mutation and inactivation of SMARCA4 (BRG1) in an atypical teratoid/rhabdoid tumor showing retained SMARCB1 (INI1) expression. Am J Surg Pathol. 2011 Jun;35(6):933-5. I.F. 4.106
-    Gessi M, Setty P, Bisceglia M, zur Muehlen A, Lauriola L, Waha A, Giangaspero  F, Pietsch T. Supratentorial primitive neuroectodermal tumors of the central nervous system in adults: molecular and histopathologic analysis of 12 cases. Am J Surg Pathol. 2011 Apr;35(4):573-82. I.F. 4.106
-    Antonelli M, Caltabiano R, Chiappetta C, Oliva MA, Giangaspero F, Lanzafame S. Primary peripheral PNET/Ewing’s sarcoma arising in the meninges, confirmed by the presence of the rare translocation t(21;22) (q22;q12). Neuropathology. 2011 Oct;31(5):549-55. doi: 10.1111/j.1440-1789.2010.01196.x. I.F. 1.605
-    Antonelli M, Hasselblatt M, Haberler C, Di Giannatale A, Garrè ML, Donofrio V, Lauriola L, Ridola V, Arcella A, Frùhwald M, Giangaspero F. Claudin-6 is of limited sensitivity and specificity for the diagnosis of atypical teratoid/rhabdoid tumors. Brain Pathol. 2011 Sep;21(5):558-63. doi:10.1111/j.1750-3639.2011.00478.x. I.F. 4.741
-    Massimino M, Giangaspero F, Garrè ML, Gandola L, Poggi G, Biassoni V, Gatta G, Rutkowski S. Childhood medulloblastoma. Crit Rev Oncol Hematol. 2011 Jul;79(1):65-83. doi: 10.1016/j.critrevonc.2010.07.010. I.F. 4.689
-    Sardi I, Massimino M, Genitori L, Buccoliero AM, Giangaspero F, Ferrari A. Intracranial mesenchymal chondrosarcoma: report of two pediatric cases. Pediatr Blood Cancer. 2011 Apr;56(4):685-6. doi: 10.1002/pbc.22906. I.F. 1.948
-    Rutkowski S, von Hoff K, Emser A, Zwiener I, Pietsch T, Figarella-Branger D,  Giangaspero F, Ellison DW, Garrè ML, Biassoni V, Grundy RG, Finlay JL, Dhall G, Raquin MA, Grill J. Survival and prognostic factors of early childhood medulloblastoma: an international meta-analysis. J Clin Oncol. 2010 Nov 20;28(33):4961-8. I.F. 18.970
-    Gasparini P, Facchinetti F, Boeri M, Lorenzetto E, Livio A, Gronchi A, Ferrari A, Massimino M, Spreafico F, Giangaspero F, Forni M, Maestro R, Alaggio R, Pilotti S, Collini P, Modena P, Sozzi G. Prognostic determinants in epithelioid sarcoma. Eur J Cancer. 2011 Jan;47(2):287-95. I.F. 4.944
-    Raz E, Antonelli M, Pichierri A, Consoli A, Giangaspero F, Fiorelli M. 35 year-old man with falcine tumor. Brain Pathol. 2010 Sep;20(5):987-8. I.F. 4.741
-    Minniti G, Salvati M, Arcella A, Buttarelli F, D’Elia A, Lanzetta G, Esposito V, Scarpino S, Maurizi Enrici R, Giangaspero F. Correlation between O6-methylguanine-DNA methyltransferase and survival in elderly patients with glioblastoma treated with radiotherapy plus concomitant and adjuvant temozolomide. J Neurooncol. 2011 Apr;102(2):311-6. I.F. 2.926
-    Ronchi A, Salaroli R, Rivetti S, Della Bella E, Di Tomaso T, Voltattorni M, Cammelli S, Ceccarelli C, Giangaspero F, Barbieri E, Cenacchi G. Lithium induces  mortality in medulloblastoma cell lines. Int J Oncol. 2010 Sep;37(3):745-52. I.F.2.571
-    Massimino M, Gandola L, Barra S, Giangaspero F, Casali C, Potepan P, Di Rocco C, Nozza P, Collini P, Viscardi E, Bertin D, Biassoni V, Cama A, Milanaccio C, Modena P, Balter R, Tamburrini G, Peretta P, Mascarin M, Scarzello G, Fidani P, Milano GM, Sardi I, Genitori L, Garrè ML. Infant ependymoma in a 10-year AIEOP (Associazione Italiana Ematologia Oncologia Pediatrica) experience with omitted or deferred radiotherapy. Int J Radiat Oncol Biol Phys. 2011 Jul 1;80(3):807-14. I.F. 4.503
-    Buttarelli FR, Massimino M, Antonelli M, Lauriola L, Nozza P, Donofrio V, Arcella A, Oliva MA, Di Rocco C, Giangaspero F. Evaluation status and prognostic  significance of O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation in pediatric high grade gliomas. Childs Nerv Syst. 2010 Aug;26(8):1051-6. I.F. 1.314
-    Massimi L, Caldarelli M, D’Alessandris QG, Rollo M, Lauriola L, Giangaspero F, Di Rocco C. 12-year-old boy with multiple brain masses. Brain Pathol. 2010 May;20(3):679-82. I.F. 4.741
-    Korshunov A, Remke M, Gessi M, Ryzhova M, Hielscher T, Witt H, Tobias V, Buccoliero AM, Sardi I, Gardiman MP, Bonnin J, Scheithauer B, Kulozik AE, Witt O, Mork S, von Deimling A, Wiestler OD, Giangaspero F, Rosenblum M, Pietsch T, Lichter P, Pfister SM. Focal genomic amplification at 19q13.42 comprises a powerful diagnostic marker for embryonal tumors with ependymoblastic rosettes. Acta Neuropathol. 2010 Aug;120(2):253-60. I.F. 7.695
-    Landi A, Piccirilli M, Mancarella C, Giangaspero F, Salvati M. Gliomatosis cerebri in young patients’ report of three cases and review of the literature. Childs Nerv Syst. 2011 Jan;27(1):19-25. I.F. 1.314
-    Iyer AM, Zurolo E, Boer K, Baayen JC, Giangaspero F, Arcella A, Di Gennaro GC, Esposito V, Spliet WG, van Rijen PC, Troost D, Gorter JA, Aronica E. Tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA) in human epileptogenic pathologies. Neuroscience. 2010 Feb 25. [Epub ahead of print] PubMed PMID: 20219643. IF.3.556
-    Massimino M, Spreafico F, Riva D, Biassoni V, Poggi G, Solero C, Gandola L, Genitori L, Modena P, Simonetti F, Potepan P, Casanova M, Meazza C, Clerici CA, Catania S, Sardi I, Giangaspero F. A lower-dose, lower-toxicity cisplatin-etoposide regimen for childhood progressive low-grade glioma. J Neurooncol. 2010 Feb 12. [Epub ahead of print] PubMed PMID: 20151174.  IF.2.323
-    Antonelli M, Buttarelli FR, Arcella A, Nobusawa S, Donofrio V, Oghaki H, Giangaspero F. Prognostic significance of histological grading, p53 status, YKL-40 expression, and IDH1 mutations in pediatric high-grade gliomas. J Neurooncol. 2010 Feb 21. [Epub ahead of print] PubMed PMID: 20174854. IF. 2.323
-    Ferretti E, De Smaele E, Po A, Di Marcotullio L, Tosi E, Espinola MS, Di Rocco C, Riccardi R, Giangaspero F, Farcomeni A, Nofroni I, Laneve P, Gioia U, Caffarelli E, Bozzoni I, Screpanti I, Gulino A. MicroRNA profiling in human medulloblastoma. Int J Cancer. 2009 Feb 1;124(3):568-77. IF. 4.734
-    Meco D, Servidei T, Riccardi A, Ferlini C, Cusano G, Zannoni GF, Giangaspero  F, Riccardi R. Antitumor effect in medulloblastoma cells by gefitinib: Ectopic HER2 overexpression enhances gefitinib effects in vivo. Neuro Oncol. 2009 Jun;11(3):250-9. IF. 5:000
-    Barresi V, Buttarelli FR, Vitarelli EE, Arcella A, Antonelli M, Giangaspero F. Caveolin-1 expression in diffuse gliomas: correlation with the proliferation index, epidermal growth factor receptor, p53, and 1p/19q status. Hum Pathol. 2009 Dec;40(12):1738-46. IF.3.322
-    Roseti C, Palma E, Martinello K, Fucile S, Morace R, Esposito V, Cantore G, Arcella A, Giangaspero F, Aronica E, Mascia A, Di Gennaro G, Quarato PP, Manfredi M, Cristalli G, Lambertucci C, Marucci G, Volpini R, Limatola C, Eusebi F. Blockage of A2A and A3 adenosine receptors decreases the desensitization of human GABA(A) receptors microtransplanted to Xenopus oocytes. Proc Natl Acad Sci U S A. 2009 Sep 15;106(37):15927-31. IF.9.380
-    Garrè ML, Cama A, Bagnasco F, Morana G, Giangaspero F, Brisigotti M, Gambini  C, Forni M, Rossi A, Haupt R, Nozza P, Barra S, Piatelli G, Viglizzo G, Capra V,  Bruno W, Pastorino L, Massimino M, Tumolo M, Fidani P, Dallorso S, Schumacher RF,Milanaccio C, Pietsch T. Medulloblastoma variants: age-dependent occurrence and relation to Gorlin syndrome–a new clinical perspective. Clin Cancer Res. 2009 Apr 1;15(7):2463-71. IF.6.488
-    Massimino M, Buttarelli FR, Antonelli M, Gandola L, Modena P, Giangaspero F.  Intracranial ependymoma: factors affecting outcome. Future Oncol. 2009 Mar;5(2):207-16.
-     Massimino M, Gandola L, Garrè ML, Cama A, Modena P, Potepan P, Giangaspero F. Do we really need class 1 evidence results to give adjuvant radiation therapy to  childhood intracranial ependymomas? Childs Nerv Syst. 2009 Jun;25(6):641-2;author reply 643-4. IF.1.351
-    Salvati M, Formichella AI, D’Elia A, Brogna C, Frati A, Giangaspero F, Delfini R, Santoro A. Cerebral glioblastoma with oligodendrogliomal component: analysis of 36 cases. J Neurooncol. 2009 Aug;94(1):129-34. IF. 2.323
-    Polli FM, Salvati M, Miscusi M, Delfini R, Giangaspero F. Neurocytoma of the  spinal cord: report of three cases and review of the literature. Acta Neurochir (Wien). 2009 Jun;151(6):569-74; discussion 574. IF.1.634
-    Antonelli M, Cenacchi G, Modena P, Morra I, Forni M, Giangaspero F. Ultrastructural evidence of ependymal differentiation in a genetically proven atypical teratoid/rhabdoid tumor. Childs Nerv Syst. 2009 Dec;25(12):1627-31. IF.1.351
-    Jaffrain-Rea ML, Angelini M, Gargano D, Tichomirowa MA, Daly AF, Vanbellinghen JF, D’Innocenzo E, Barlier A, Giangaspero F, Esposito V, Ventura L, Arcella A, Theodoropoulou M, Naves LA, Fajardo C, Zacharieva S, Rohmer V, Brue T, Gulino A, Cantore G, Alesse E, Beckers A. Expression of aryl hydrocarbon receptor (AHR) and AHR-interacting protein in pituitary adenomas: pathological and clinical implications. Endocr Relat Cancer. 2009 Sep;16(3):1029-43. IF. 5.236
-    Pettorini BL, Novegno F, Cianfoni A, Massimi L, De Bonis P, Esposito G, Caldarelli M, Tamburrini G, Di Rocco C, Giangaspero F, Lauriola L. 5-year-old boy with a clival mass. Brain Pathol. 2009 Jul;19(3):523-6. IF.5.576
-    Antonelli M, Acerno S, Baldoli C, Terreni MR, Giangaspero F. A case of melanotic desmoplastic ganglioglioma. Neuropathology. 2009 Oct;29(5):597-601. IF.1.727
-    Russo N, Giangaspero F, Beccaglia MR, Santoro A. Intracranial dural histiocytosis. Br J Neurosurg. 2009 Aug;23(4):449-54. IF.0.764
-    Servidei T, Meco D, Riccardi A, Ferlini C, Cusano G, Zannoni GF, Giangaspero F, Riccardi R. Antitumor effect in medulloblastoma cells by gefitinib: Ectopic HER2 overexpression enhances gefitinib effects in vivo. Neuro Oncol 2009, 11(3):250-9. IF.5.000
-    Salvati M, D’Elia A, Brogna C, Frati A, Antonelli M, Giangaspero F, Raco A, Santoro A, Delfini R. Cerebral astroblastoma: analysis of six cases and critical  review of treatment options. J Neurooncol. 2009 Jul;93(3):369-78. IF.2.323
-    Gandola L, Massimino M, Cefalo G, Solero C, Spreafico F, Pecori E, Riva D, Collini P, Pignoli E, Giangaspero F, Luksch R, Berretta S, Poggi G, Biassoni V, Ferrari A, Pollo B, Favre C, Sardi I, Terenziani M, Fossati-Bellani F. Hyperfractionated accelerated radiotherapy in the Milan strategy for metastatic medulloblastoma. J Clin Oncol. 2009;27(4):566-71. IF.17.157
-    Ciceroni C, Arcella A, Mosillo P, Battaglia G, Mastrantoni E, Oliva MA, Carpinelli G, Santoro F, Sale P, Ricci-Vitiani L, De Maria R, Pallini R, Giangaspero F, Nicoletti F, Melchiorri D. Type-3 metabotropic glutamate receptors negatively modulate bone morphogenetic protein receptor signaling and support the tumourigenic potential of glioma-initiating cells .Neuropharmacology. 2008, 55(4):568-76. IF.3.383
-    Salaroli R, Di Tomaso T, Ronchi A, Ceccarelli C, Cammelli S, Cappellini A, Martinelli GN, Barbieri E, Giangaspero F, Cenacchi G. Radiobiologic response of medulloblastoma cell lines: involvement of beta-catenin? J Neurooncol. 2008 Dec;90(3):243-51. IF.2.323
-    di Norcia V, Piccirilli M, Giangaspero F, Salvati M. Gliosarcomas in the elderly: analysis of 7 cases and clinico-pathological remarks. Tumori. 2008 Jul-Aug;94(4):493-6. IF. 0.791
-    Gessi M,Giangaspero F, Lauriola L. ,Gardiman M, Scheithauer BW, Halliday W, Hawkins C,  Rosenblum MK, Burger PC, , Eberhart CG  Embryonal tumors with abundant neuropil and true rosettes: a distinctive CNS primitive neuroectodermal tumor. Am J Surg Pathol. 2009 Feb;33(2):211-7. IF.4.535
-    Tanori M, Mancuso M, Pasquali E, Leonardi S, Rebessi S, Di Majo V, Guilly MN,  Giangaspero F, Covelli V, Pazzaglia S, Saran A. PARP-1 cooperates with Ptc1 to suppress medulloblastoma and basal cell carcinoma. Carcinogenesis. 2008 Oct;29(10):1911-9. IF.4.930
-    Gulino A, Arcella A, Giangaspero F. Pathological and molecular heterogeneity of  medulloblastoma. Curr Opin Onc 2008, 20:668-675. IF.4.116
-    Piccirilli M, Brunetto GM, Rocchi G, Giangaspero F, Salvati M. Extra central nervous system metastases from cerebral glioblastoma multiforme in  elderly patients. Clinico-pathological remarks on our series of seven cases and critical review of the literature. Tumori. 2008 Jan-Feb;94(1):40-5. IF. 0.791
-    Denaro L, Gardiman M, Calderone M, Rossetto M, Ciccarino P, Giangaspero F, Perilongo G, d’Avella D. Intraventricular astroblastoma. Case report. J Neurosurg Pediatrics. 2008 Feb;1(2):152-5. IF.–
-    Pezzolo A, Capra V, Raso A, Morandi F, Parodi F, Gambini C, Nozza P, Giangaspero F, Cama A, Pistoia V, Garrè ML. Identification of novel chromosomal abnormalities and prognostic cytogenetics markers in intracranial pediatric ependymoma. Cancer Lett. 2008 Mar 18;261(2):235-43. IF.3.504
-    Donato V, Papaleo A, Castrichino A, Banelli E, Giangaspero F, Salvati M, Delfini R. Prognostic implication of clinical and pathologic features in patients with glioblastoma multiforme treated with concomitant radiation plus temozolomide. Tumori. 2007 May-Jun;93(3):248-56. IF.0.791
-    Gessi M, Monego G, Calviello G, Lanza P, Giangaspero F, Silvestrini A, Lauriola L, Ranelletti FO.  Human parathyroid hormone-related protein and human parathyroid hormone receptor  type 1 are expressed in human medulloblastomas and regulate cell proliferation and apoptosis in medulloblastoma-derived cell lines. Acta Neuropathol. 2007 Aug;114(2):135. IF.5.310
-    Salaroli R, Russo A, Ceccarelli C, Mina GD, Arcella A, Martinelli GN, Giangaspero F, Capranico G, Cenacchi G. Intracellular distribution of beta-catenin in human medulloblastoma cell lines with different degree of neuronal differentiation. Ultrastruct Pathol. 2007 Jan-Feb;31(1):33-44. IF.0.768
-    Piccirilli M, Sassun TE, Brogna C, Giangaspero F, Salvati M. Late brain metastases from breast cancer: clinical remarks on 11 patients and review of the literature. Tumori. 2007 Mar-Apr;93(2):150-4. IF.0.791
-    Nicoletti F, Arcella A, Iacovelli L, Battaglia G, Giangaspero F, Melchiorri D. Metabotropic glutamate receptors: new targets for the control of tumor growth? Trends Pharmacol Sci. 2007 May;28(5):206-13. IF.9.340
-    Amantini C, Mosca M, Nabissi M, Lucciarini R, Caprodossi S, Arcella A, Giangaspero F, Santoni G. Capsaicin-induced apoptosis of glioma cells is mediated by TRPV1 vanilloid receptor and requires p38 MAPK activation. J Neurochem. 2007 Aug;102(3):977-90. IF.4.500
-    Kros JM, Gorlia T, Kouwenhoven MC, Zheng PP, Collins VP, Figarella-Branger D,  Giangaspero F, Giannini C, Mokhtari K, Mørk SJ, Paetau A, Reifenberger G, van den Bent MJ.  Panel review of anaplastic oligodendroglioma from European Organization For Research and Treatment of Cancer Trial 26951: assessment of consensus in diagnosis, influence of 1p/19q loss, and correlations with outcome. J Neuropathol Exp Neurol. 2007 Jun; 66(6):545-51. IF.5.140
-    Fratticci A, Grieco FA, Spilioti C, Giangaspero F, Ventura L, Esposito V, Piccirilli M, Santoro A, Gulino A, Cantore G, Alesse E, Jaffrain-Rea ML.  Differential expression of neurogenins and NeuroD1 in human pituitary tumours. J Endocrinol. 2007 Sep;194(3):475-84. IF.2.791
-    Modena P, Lualdi E, Facchinetti F, Veltman J, Reid JF, Minardi S, Janssen I, Giangaspero F, Forni M, Finocchiaro G, Genitori L, Giordano F, Riccardi R, Schoenmakers EF, Massimino M, Sozzi G. Identification of tumor-specific molecular signatures in intracranial ependymoma and association with clinical characteristics. J Clin Oncol. 2006 Nov 20;24(33):5223-33. IF.17.157
-    Visentini M, Carbonari M, Ghia E, De Propriis S, Guarini A, Girmenia C,Giannini G, Sabattini E, Ceccarini C, Zamarchi R, Giangaspero F, Novelli A, Amadori A, Pileri SA, Fiorilli M. A lymphotactin-producing monoclonal T-cell lymphoproliferative disorder with extreme lymphocytopenia and progressive leukoencephalopathy. Leuk Lymphoma. 2006 Jul;47(7):1421-3. IF. 1.939
-    Bruschi F, Giangaspero F, Castagna M, Becherini F, Filice ME, Vannozzi R, Cantini R. Neurocysticercosis: surgical treatment of an autochthonous case in a non-endemic region. Pathologica. 2006 Aug;98(4):229-31. IF–
-    Iacovelli L, Arcella A, Battaglia G, Pazzaglia S, Aronica E, Spinsanti P, Caruso A, De Smaele E, Saran A, Gulino A, D’Onofrio M, Giangaspero F, Nicoletti F.  Pharmacological activation of mGlu4 metabotropic glutamate receptors inhibits the growth of medulloblastomas. J Neurosci. 2006 Aug 9;26(32):8388-97. IF.7.452
-    Ferretti E, Di Marcotullio L, Gessi M, Mattei T, Greco A, Po A, De Smaele E, Giangaspero F, Riccardi R, Di Rocco C, Pazzaglia S, Maroder M, Alimandi M, Screpanti I, Gulino A.  Alternative splicing of the ErbB-4 cytoplasmic domain and its regulation by hedgehog signaling identify distinct medulloblastoma subsets. Oncogene. 2006, 25(55):7267-73. IF.7.216
-    Massimino M, Giangaspero F, Garre ML, Genitori L, Perilongo G, Collini P, Riva D, Valentini L, Scarzello G, Poggi G, Spreafico F, Peretta P, Mascarin M, Modena P, Sozzi G, Bedini N, Biassoni V, Urgesi A, Balestrini MR, Finocchiaro G, Sandri A, Gandola L; AIEOP Neuro-Oncology Group.  Salvage treatment for childhood ependymoma after surgery only: Pitfalls of omitting “at once” adjuvant treatment. Int J Radiat Oncol Biol Phys. 2006 Aug 1;65(5):1440-5. IF.4.639
-    Massimino M, Gandola L, Spreafico F, Biassoni V, Terenziani M, Pecori E, Arcella A, Giangaspero F.  Unusual primary secreting germ cell tumor of the spine. Case report. J Neurosurg Spine. 2006 Jul;5(1):65-7. IF.1.403
-    Piccirilli M, Bistazzoni S, Gagliardi FM, Landi A, Santoro A, Giangaspero F, Salvati M.  Treatment of glioblastoma multiforme in elderly patients. Clinico-therapeutic remarks in 22 patients older than 80 years. Tumori. 2006 Mar-Apr;92(2):98-103. IF.0.791
-    Celli P, Caroli E, Giangaspero F, Ferrante L.  Papillary Glioneuronal Tumor : Case Report and Literature Review. J Neurooncol. 2006… IF.2.323
-    Rossi A, Garre ML, Ravegnani M, Nozza P, Abbruzzese A, Giangaspero F, Tortori-Donati P.  Bilateral germinoma of the basal ganglia. Pediatr Blood Cancer. 50(1):177-9. IF.2. 394
-    Giangaspero F, Wellek S, Masuoka J, Gessi M, Kleihues P, Ohgaki H.  Stratification of medulloblastoma on the basis of histopathological grading. Acta Neuropathol (Berl). 2006 Jul;112(1):5-12. IF.7.216
-    Pazzaglia S, Tanori M, Mancuso M, Gessi M, Pasquali E, Leonardi S, Oliva MA, Rebessi S, Di Majo V, Covelli V, Giangaspero F, Saran A.  Two-hit model for progression of medulloblastoma preneoplasia in Patched heterozygous mice. Oncogene. 2006 Sep 7;25(40):5575-80. IF.7216
-    Salvati M, Lenzi J, Brogna C, Frati A, Piccirilli M, Giangaspero F, Raco A.  Childhood’s gliosarcomas: pathological and therapeutical considerations on three cases and critical review of the literature. Childs Nerv Syst. 2006 Oct;22(10):1301-6. IF.1.351
-    Massimino M, Gandola L, Spreafico F, Luksch R, Collini P, Giangaspero F, Simonetti F, Casanova M, Cefalo G, Pignoli E, Ferrari A, Terenziani M, Podda M, Meazza C, Polastri D, Poggi G, Ravagnani F, Fossati-Bellani F.   Supratentorial primitive neuroectodermal tumors (S-PNET) in children: A prospective experience with adjuvant intensive chemotherapy and hyperfractionated accelerated radiotherapy. Int J Radiat Oncol Biol Phys. 2006 Mar 15;64(4):1031-7. Epub 2005 Dec 15. IF.4.639
-    Ragozzino D, Palma E, Di Angelantonio S, Amici M, Mascia A, Arcella A, Giangaspero F, Cantore G, Di Gennaro G, Manfredi M, Esposito V, Quarato PP, Miledi R, Eusebi F. Rundown of GABA type A receptors is a dysfunction associated with human drug-resistant mesial temporal lobe epilepsy. Proc Natl Acad Sci U S A. 2005 Oct 18;102(42):15219-23. IF.9.64
-    Piccirilli M, Salvati M, Bistazzoni S, Frati A, Brogna C, Giangaspero F, Frati R, Santoro A. Glioblastoma multiforme and breast cancer: report on 11 cases and clinico-pathological remarks. Tumori. 2005 May-Jun;91(3):256-60. IF.0.791
-    Sandri A, Massimino M, Mastrodicasa L, Sardi N, Bertin D, Basso ME, Todisco L, Paglino A, Perilongo G, Genitori L, Valentini L, Ricardi U, Gandola L, Giangaspero F, Madon E. Treatment with oral etoposide for childhood recurrent ependymomas. J Pediatr Hematol Oncol. 2005 Sep;27(9):486-90. IF.1.176
-    Caroli E, Salvati M, Giangaspero F, Ferrante L, Santoro A. Intrameningioma metastasis as first clinical manifestation of occult primari breast carcinoma. Neurosurg Rev. 2006 Jan;29(1):49-54. IF.1.727
-    Salvati M, Caroli E, Frati A, Piccirilli M, Agrillo A, Brogna C, Occhiogrosso G, Giangaspero F. Central nervous system mesenchymal chondrosarcoma. J Exp Clin Cancer Res. 2005 Jun;24(2):317-24. IF.1.184
-    Salvati M, Caroli E, Raco A, Giangaspero F, Delfini R, Ferrante L. Gliosarcomas: analysis of 11 cases do two subtypes exist? J Neurooncol. 2005 Aug;74(1):59-63. IF.2.323
-    Arcella A, Carpinelli G, Battaglia G, D’Onofrio M, Santoro F, Ngomba RT, Bruno V, Casolini P, Giangaspero F, Nicoletti F. Pharmacological blockade of group II metabotropic glutamate receptors reduces the growth of glioma cells in vivo. Neuro-oncol. 2005 Jul;7(3):236-45. IF.5.000
-    Salvati M, Caroli E, Paone C, Frati A, Ferrante L, Giangaspero F, Delfini R. Brain metastasis from cutaneous squamous cell carcinoma of the dorsum. Case report. J Neurosurg. 2005 Jun;102(6):1155-8. IF.2.124
-    Lenzi J, Salvati M, Frati A, Raco A, Pichierri A, Giangaspero F, Delfini R. Intraventricular neurocytoma with massive brain stem involvement in a 5-year-old child. Childs Nerv Syst. 2006,22(1):95-8. IF.1.351
-    Massimino M, Gandola L, Luksch R, Spreafico F, Riva R, Carlo Solero, Giangaspero F, Locatelli F, Podda M, Bozzi F, Pignoli E, Collini P, Cefalo G, Zecca M, et al. Sequential chemotherapy, high-dose thiotepa, circulating progenitor cell rescue, and radiotherapy for childhood high-grade glioma. Neuro-Oncology  2005; 7: 41-48. IF.5.000
-    Gessi M, Marani C, Geddes J, Arcella A, Cenacchi G, Giangaspero F. Ependymoma with neuropil-like islands: a case report with diagnostic and histogenetic implications. Acta Neuropathol (Berl). 2005; 109: 231-234. IF.5.310
-    Horstmann S, Perry A, Reifenberger G, Giangaspero F et al. Genetic and expression profiles of cerebellar liponeurocytomas.Brain Pathol. 2004 Jul;14(3):281-9. IF.5.576
-    Canudas AM, Di Giorgi-Gerevini V, Iacovelli L, Nano G, D’Onofrio M, Arcella A, Giangaspero F, Busceti C, Ricci-Vitiani L, Battaglia G, Nicoletti F, Melchiorri D. PHCCC, a specific enhancer of mGlu4 metabotropic glutamate receptors, reduces proliferation and promotes differentiation of cerebellar granule cell neuroprecursors. J Neurosci 2004; 24:10343-10352 . IF.7.452
-    Di Marcotullio L, Ferretti E, De Smaele E, Argenti B, Mincione C, Zazzeroni F, Gallo R, Masuelli L, Napolitano M, Maroder M, Modesti A, Giangaspero F,Screpanti I, Alesse E, Gulino A.  REN(KCTD11) is a suppressor of Hedgehog signaling and is deleted in human edulloblastoma. Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10833-8. IF.9.380
-    Caroli E, Salvati M, Esposito V, Orlando ER, Giangaspero F.  Cerebral astroblastoma. Acta Neurochir (Wien). 2004 Jun;146(6):629-33. IF.1.634
-    Caroli E, Salvati M, Orlando ER, Lenzi J, Santoro A, Giangaspero F.  Solitary fibrous tumors of the meninges: report of four cases and literature eview. Nurosurg Rev. 2004 Oct;27(4):246-51. IF.1.727
-    Massimino M, Gandola L, Giangaspero F et al. Hyperfractionated radiotherapy and chemotherapy for childhood ependymoma: final results of the first prospective AIEOP (Associazione Italiana di Ematologia-Oncologia Pediatrica) study. Int J Radiat Oncol Biol Phys. 2004 Apr 1;58(5):1336-45. IF.4.639
-    Salvati M, Caroli E, Orlando ER, Nardone A, Frati A, Innocenzi G, Giangaspero F.  Solitary brain metastases from uterus carcinoma: report of three cases. J Neurooncol. 2003 Jan;66(1-2):175-8. IF.2.323
-    Cenacchi G, Giangaspero F. Emerging tumor entities and variants of CNS neoplasms. J Neuropathol Exp Neurol 2004 63:185-92. IF.5.140
-    Gessi M, Giangaspero F, Pietsch T. Atypical teratoid/rhabdoid tumors and choroid plexus tumors: when genetics “surprise” pathology. Brain Pathol. 2003 Jul;13(3):409-14. IF.5.576
-    Giangaspero F. [CNS embryonal tumors: pathology and genetics] Pathologica. 2003 95(5):237-9. IF—-
-    Armstrong DD, Giangaspero F. Pediatric brain tumors: introduction. Brain Pathol. 2003 Jul;13(3):373-5. IF.5.576
-    Ferraraccio F, Accardo M, Giangaspero F, Cuccurullo L.  Recurrent and atypical meningiomas–a multiparametric study using Ki67 labelling index, AgNOR and DNA Feulgen staining. Clin Neuropathol. 2003 Jul-Aug;22(4):187-92.  IF.1.200
-    Caroli E, Salvati M, Peruzzi P, Frati A, Giangaspero F.  Familial gliomas. Analysis of six families with cerebral gliomas and without other inheritable syndromes.Neurosurg Rev. 2003 Oct;26(4):280-2. IF.1.727
-    Tamburrini G, Colosimo C Jr, Giangaspero F, Riccardi R, Di Rocco C. Desmoplastic infantile ganglioglioma.Childs Nerv Syst. 2003 Jun;19(5-6):292-7.
-    Tamburrini G, Gessi M, Colosimo C Jr, Lauriola L, Giangaspero F, Di Rocco C. Infantile myofibromatosis of the central nervous system. Childs Nerv Syst. 2003 Sep;19(9):650-4. IF.1.351
-    Perilongo G, Garre ML, Giangaspero F. Low-grade gliomas and leptomeningeal dissemination: a poorly understood phenomenon.Childs Nerv Syst. 2003 Apr;19(4):197-203. IF. 1.351
-    La Spina M, Dollo C, Giangaspero F, Bertolini P, Russo G. Intracranial mesenchymal chondrosarcoma with osteoid formation: report of a pediatric case. Childs Nerv Syst. 2003 Sep;19(9):680-2. IF. 1.351
-    Vincent S, Mirshari M, Nicolas C, Adenis C, Dhellemmes P, Soto Ares G, Maurage CA, Baranzelli MC, Giangaspero F, Ruchoux MM.  Large-cell medulloblastoma with arrestin-like protein expression. Clin Neuropathol. 2003 Jan-Feb;22(1):1-9. IF.1.200
-    Palma E, Esposito V, Mileo AM, Di Gennaro G, Quarato P, Giangaspero F, Scoppetta C, Onorati P, Trettel F, Miledi R, Eusebi F.  Expression of human epileptic temporal lobe neurotransmitter receptors in Xenopus oocytes: An innovative approach to study epilepsy. Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):15078-83. IF.9.340
-    Perilongo G, Gardiman M, Bisaglia L, Rigobello L, Calderone M, Battistella A, Burnelli R, Giangaspero F.  Spinal low-grade neoplasms with extensive leptomeningeal dissemination in children. Childs Nerv Syst. 2002 Oct;18(9-10):505-12. IF.1.351
-    Riccioni L, Farabegoli P, Nanni O, Morigi F, Polverelli M, Landi G, Giangaspero F. The sentinel lymph node in melanoma: utilization of molecular biology (RT-PCR) to detect occult metastases] Pathologica. 2002 Aug;94(4):190-5. IF-
-    Giangaspero F, Kaulich K, Cenacchi G, Cerasoli S, Lerch KD, Breu H, Reuter T, Reifenberger G. Lipoastrocytoma: a rare low-grade astrocytoma variant of pediatric age. Acta Neuropathol (Berl). 2002 Feb;103(2):152-6. IF.5.310
-    Cenacchi G, Giovenali P, Castrioto C, Giangaspero F.  Pituicytoma: ultrastructural evidence of a possible origin from folliculo-stellate cells of the adenohypophysis. Ultrastruct Pathol. 2001 Jul-Aug;25(4):309-12.  IF.0.768
-    Cerasoli S, Spada F, Buda R, Turci A, Giangaspero F.  [Cytogenetic analysis of 19 renal cell tumors] Pathologica. 2001 Apr;93(2):118-23. Italian.  IF.–
-    Steigerwald K, Santoro IM, Kordich JJ, Gismondi V, Trzepacz C, Badiali M, Giangaspero F, Balko MG, Graham JS, Ratner N, Lowy AM, Varesco L, Groden J.  A distinct splice form of APC is highly expressed      in neurones but not commonly mutated in      neuroepithelial tumours. J Med Genet. 2001 Apr;38(4):257-62. IF.5.713
-    Fraggetta F, Galia A, Grasso G, D’Arrigo C, Cristaudo C, Giangaspero F. Pulmonary adenocarcinoma metastatic to pituitary craniopharyngioma.J Clin Pathol. 2000 Dec;53(12):946- IF.2.342
-    Bisceglia M, D’Angelo V, Parisi S, Lelli G, Pasquinelli G, Cenacchi G, Giangaspero F.  [Primary tumors of the central nervous system: histogenetic classification, grade of malignity, clinico-pathologic features, and therapeutic criteria] Pathologica. 2000 Dec;92(6):534-61. IF–
-    Cenacchi G, Roncaroli F, Cerasoli S, Ficarra G, Merli GA, Giangaspero F.  Chordoid glioma of the third ventricle: an ultrastructural study of three cases with a histogenetic hypothesis. Am J Surg Pathol. 2001 Mar;25(3):401-5. IF.4.535
-    Pennella A, Ferri C, Lettini T, Serio G, Cimmino A, Caniglia DM, Giangaspero F, Ricco R.  [Atypical meningioma: morphometric analysis of nuclear pleomorphism] Pathologica. 2000 Feb;92(1):25-31.
-    Cenacchi G, Roncaroli F, Cerasoli S, Ficarra G, Giangaspero F.  [Chordoid tumor of the 3d ventricle: immunohistochemical and ultrastructural study of 3 cases] Pathologica. 2000 Apr;92(2):132-3.
-    Cimmino A, Giangaspero F, Pennella A, Serio G, De Tomasi A, Colamaria A, Ricco R. [Oncocytic meningioma. Case report] Pathologica. 2000 Apr;92(2):82-5.
-    Lucchi I, Morigi F, Naldi S, Tabarri B, Elegibili E, Giangaspero F.  [Cell-block immunocytochemical characterization of effusions. Use of antibody panel: calretinin, Ber-EP4, keratin and CD68] Pathologica. 1999 Dec;91(6):447-52.
-    Loda M, Xu X, Pession A, Vortmeyer A, Giangaspero F. Membranous expression of glucose transporter-1 protein (GLUT-1) in embryonal neoplasms of the central nervous system. Neuropathol Appl Neurobiol. 2000 Feb;26(1):91-7. IF.3.666
-    Riccioni L, Morigi F, Naldi S, Polverelli M, Morelli R, Fiscelli O, Landi G, Giangaspero F.
-    [Anatomo-pathologic study of sentinel lymph nodes in melanoma. Analysis of 200 cases] Pathologica. 1999 Aug;91(4):242-8.
-    Giangaspero F, Perilongo G, Fondelli MP, Brisigotti M, Carollo C, Burnelli R, Burger PC, Garre ML.  Medulloblastoma with extensive nodularity: a variant with favorable prognosis. J Neurosurg. 1999 Dec;91(6):971-7.  IF.2.124
-    Cenacchi G, Morra I, Forni M, Giangaspero F.  Mucin-secreting cellular ependymoma: a light and electron microscopy study. Ultrastruct Pathol. 1999 Sep-Oct;23(5):319-23.  IF.0.768
-    Giangaspero F, Cenacchi G.  Oncocytic and granular cell neoplasms of the central nervous system and pituitary gland. Semin Diagn Pathol. 1999 May;16(2):91-7. IF.3.136
-    Giangaspero F, Guiducci A, Lenz FA, Mastronardi L, Burger PC.  Meningioma with meningioangiomatosis: a condition mimicking invasive meningiomas in children and young adults: report of two cases and review of the literature. Am J Surg Pathol. 1999 Aug;23(8):872-5. IF.4.535
-    Brunori A, Cerasoli S, Donati R, Giangaspero F, Chiappetta F.  Solitary fibrous tumor of the meninges: two new cases and review of the literature. Surg Neurol. 1999 Jun;51(6):636-40. IF.1.556
-    Casadei GP, Giangaspero F.  Cerebrospinal lesions simulating central nervous system neoplasm. Adv Clin Path. 1998 Jan;2(1):25-35.  IF.–
-    Mascalchi M, Simonelli P, Tessa C, Giangaspero F, Petruzzi P, Bosincu L, Conti M, Sechi G, Salvi F.  Do acute lesions of Wernicke’s encephalopathy show contrast enhancement? Report of three cases and review of the literature. Neuroradiology. 1999 Apr;41(4):249-54. IF.2.029
-    Calista D, Boschini A, Giangaspero F.  Guess What! Localised bacillary angiomatosis of the tongue. Eur J Dermatol. 1998 Jun;8(4):283-4. IF.1.968
-    Bisceglia M, Sickel JZ, Giangaspero F, Gomes V, Amini M, Michal M. Littoral cell angioma of the spleen: an additional report of four cases with emphasis on the association with visceral organ cancers. Tumori. 1998 Sep-Oct;84(5):595-9. IF.0.791
-    Murgia A, Anglani F, Vinanzi C, Polli R, Basso G, Perilongo G, Giangaspero F, Zacchello F. The guanine triphosphatase (GTPase) activating protein (GAP)-related domain of the neurofibromatosis type 1 gene is not mutated in neural crest-derived sporadic tumours. Eur J Cancer. 1998 Mar;34(4):577-9. IF.4.475
-    Cenacchi G, Pasquinelli G, Montanaro L, Cerasoli S, Vici M, Bisceglia M, Giangaspero F, Martinelli GN, Derenzini M. Primary endocervical extraosseous Ewing’s sarcoma/PNET. Int J Gynecol Pathol. 1998 Jan;17(1):83-8. IF.1.766
-    Roncaroli F, Giangaspero F, Piana S, Andreoli A, Ricci R. Low-grade adenocarcinoma of endolymphatic sac mimicking jugular paraganglioma at clinical and neuroradiological examination. Clin Neuropathol. 1997 Sep-Oct;16(5):243-6. IF.1.200
-    Perilongo G, Massimino M, Sotti G, Belfontali T, Masiero L, Rigobello L, Garre L, Carli M, Lombardi F, Solero C, Sainati L, Canale V, del Prever AB, Giangaspero F, Andreussi L, Mazza C, Madon E. Analyses of prognostic factors in a retrospective review of 92 children with ependymoma: Italian Pediatric Neuro-oncology Group. Med Pediatr Oncol. 1997 Aug;29(2):79-85. IF—
-    Giangaspero F, Cenacchi G, Losi L, Cerasoli S, Bisceglia M, Burger PC. Extraventricular neoplasms with neurocytoma features. A clinicopathological study of 11 cases. Am J Surg Pathol. 1997 Feb;21(2):206-12. IF.4.535
-    Taruscio D, Danesi R, Montaldi A, Cerasoli S, Cenacchi G, Giangaspero F. Nonrandom gain of chromosome 7 in central neurocytoma: a chromosomal analysis and fluorescence in situ hybridization study. Virchows Arch. 1997 Jan;430(1):47-51. IF2.082
-    Scarani P, Neroni S, Giangaspero F, Orcioni GF, Eusebi V.  [Carlo Martinotti: the real discoverer of Martinotti's cells] Pathologica. 1996 Dec;88(6):506-10. Italian.
-    Cenacchi G, Giangaspero F, Cerasoli S, Manetto V, Martinelli GN. Ultrastructural characterization of oligodendroglial-like cells in central nervous system tumors. Ultrastruct Pathol. 1996 Nov-Dec;20(6):537-47. IF.0.768
-    Pozzati E, Giangaspero F, Marliani F, Acciarri N. Occult cerebrovascular malformations after irradiation. Neurosurgery. 1996 Oct;39(4):677-82; discussion 682-4. IF.3.398
-    Roncaroli F, Giangaspero F. [Treatment of tissues and specimens in case of suspected spongiform encephalopathy (prion's disease)] Pathologica. 1996 Jun;88(3):241-2. Italian. No abstract available.
-    Giangaspero F, Cenacchi G, Roncaroli F, Rigobello L, Manetto V, Gambacorta M, Allegranza A. Medullocytoma (lipidized medulloblastoma). A cerebellar neoplasm of adults with favorable prognosis. Am J Surg Pathol. 1996 Jun;20(6):656-64. IF. 4.535
-    Pozzati E, Acciarri N, Tognetti F, Marliani F, Giangaspero F.  Growth, subsequent bleeding, and de novo appearance of cerebral cavernous angiomas. Neurosurgery. 1996 Apr;38(4):662-9; discussion 669-70. IF.3.398
-    Montine TJ, O’Keane JC, Eskin TA, Giangaspero F, Gray L, Friedman AH, Burger PC.  Vascular malformations presenting as spinal cord neoplasms: case report. Neurosurgery. 1995 Jan;36(1):194-7; discussion 197-8. IF.3.398
-    Alampi G, Baroni R, Berti E, Ceccarelli C, Dina R, Eusebi V, Giangaspero F, Grigioni FW, Lecce S, Losi L, et al. [Quality control at the Istituto di Anatomia e Istologia patologica at the Universita di Bologna] Pathologica. 1994 Apr;86(2):184-90. Italian.
-    Albrecht S, von Deimling A, Pietsch T, Giangaspero F, Brandner S, Kleihues P, Wiestler OD. Microsatellite analysis of loss of heterozygosity on chromosomes 9q, 11p and 17p in medulloblastomas. Neuropathol Appl Neurobiol. 1994 Feb;20(1):74-81. IF. 3.666
-    Badiali M, Iolascon A, Loda M, Scheithauer BW, Basso G, Trentini GP, Giangaspero F. p53 gene mutations in medulloblastoma. Immunohistochemistry, gel shift analysis, and sequencing. Diagn Mol Pathol. 1993 Mar;2(1):23-8. IF.1.770
-    Pirini MG, Giangaspero F. [Pathologic aspects of viral infections of the central nervous system in childhood] Minerva Pediatr. 1992 Oct;44(10 Suppl 1):137-40. Review. Italian. No abstract available.
-    Giangaspero F, Rigobello L, Badiali M, Loda M, Andreini L, Basso G, Zorzi F, Montaldi A.  Large-cell medulloblastomas. A distinct variant with highly aggressive behavior. Am J Surg Pathol. 1992 Jul;16(7):687-93. IF. 4.535
-    Sainati L, Montaldi A, Putti MC, Giangaspero F, Rigobello L, Stella M, Zanesco L, Basso G. Cytogenetic t(11;17)(q13;q21) in a pediatric ependymoma. Is 11q13 a recurring breakpoint in ependymomas? Cancer Genet Cytogenet. 1992 Apr;59(2):213-6. IF.1.482
-    Loda M, Giangaspero F, Badiali M, Capodieci P, Pession A. p53 gene expression in medulloblastoma by quantitative polymerase chain reaction. Diagn Mol Pathol. 1992 Mar;1(1):36-44. IF.1.770
-    Casadei GP, Arrigoni GL, Versari P, Gambacorta M, Giangaspero F.  Central neurocytoma. A clinico-pathologic study of five cases. Tumori. 1991 Aug 31;77(4):323-7. IF.0.791
-    Giangaspero F, Pession A, Trere D, Badiali M, Galassi E, Ceccarelli C, Cavazzana A, Betts CM, Paolucci P, Stella M, et al. Establishment of a human medulloblastoma cell line (BO- 01) demonstrating skeletal muscle differentiation. Tumori. 1991 Jun 30;77(3):196-205. IF.0.791
-    Andreini L, Doglioni C, Giangaspero F.  Tubular adenoma of choroid plexus: a case report. Clin Neuropathol. 1991 May-Jun;10(3):137-40. IF,1.200
-    Badiali M, Pession A, Basso G, Andreini L, Rigobello L, Galassi E, Giangaspero F.  N-myc and c-myc oncogenes amplification in medulloblastomas. Evidence of particularly aggressive behavior of a tumor with c-myc amplification. Tumori. 1991 Apr 30;77(2):118-21. IF0.791
-    Iolascon A, Lania A, Badiali M, Pession A, Saglio G, Giangaspero F, Miraglia del Giudice E, Perrotta S, Cutillo S.  Analysis of N-ras gene mutations in medulloblastomas by polymerase chain reaction and oligonucleotide probes in formalin-fixed, paraffin-embedded tissues. Med Pediatr Oncol. 1991;19(4):240-5. IF–
-    Budka H, Wiley CA, Kleihues P. Artigas J, Asbury AK, Cho ES,  Cornblath DR, Del Canto MC, DeGirolami U, Dickson D, Epstein LG, Esiri MM, Giangaspero F, et al. HIV-associated disease of the nervous system: review of nomenclature and proposal for neuropathology-based terminology. Brain Pathol 1991;1.143-152. IF. 5.576
-    Giangaspero F, Chieco P, Ceccarelli C, Lisignoli G, Pozzuoli R, Gambacorta M, Rossi G, Burger PC.  “Desmoplastic” versus “classic” medulloblastoma: comparison of DNA content,histopathology and differentiation. Virchows Arch A Pathol Anat Histopathol. 1991;418(3):207-14. IF.2.082
-    Galliani I, Falcieri E, Giangaspero F, Valdre G, Mongiorgi R. A preliminary study of human pineal gland concretions: structural and chemical analysis.Boll Soc Ital Biol Sper. 1990 Jul;66(7):615-22. IF–
-    Alampi G, Bortolotti M, Mattioli S, Giangaspero F, Rossi L. Brain stem encephalitis in a patient with gastroduodenal and cardiovascular dysfunction: a case report. Clin Neuropathol. 1990 Jan-Feb;9(1):16-20. IF.1200
-    Galliani I, Frank F, Gobbi P, Giangaspero F, Falcieri E.  Histochemical and ultrastructural study of the human pineal gland in the course of aging. J Submicrosc Cytol Pathol. 1989 Jul;21(3):571-8.
-    Giangaspero F, Scanabissi E, Baldacci MC, Betts CM.  Massive neuronal destruction in human immunodeficiency virus (HIV) encephalitis. A clinico-pathological study of a pediatric case. Acta Neuropathol (Berl). 1989;78(6):662-5. IF.5.310
-    Giangaspero F, Fratamico FC, Ceccarelli C, Brisigotti M. Malignant peripheral nerve sheath tumors and spindle cell sarcomas: an immunohistochemical analysis of multiple markers. Appl Pathol. 1989;7(2):134-44. IF–
-    Giangaspero F, Foschini MP. Diffuse axonal swellings in a case of acquired immunodeficiency syndrome.Arch Pathol Lab Med. 1988 Dec;112(12):1259-62. IF.2.527
-    Giangaspero F, Schiavina M, Sturani C, Mondini S, Cirignotta F. Failure of automatic control of ventilation (Ondine’s curse) associated with viral encephalitis of the brainstem: a clinicopathologic study of one case. Clin Neuropathol. 1988 Sep-Oct;7(5):234-7. IF.1.200
-    Mancini AM, Giangaspero F. On large, intracytoplasmic bodies.Am J Clin Pathol. 1988 Jul;90(1):119. IF.2.715
-    Boekelheide K, Anthony DC, Giangaspero F, Gottfried MR, Graham DG. Aliphatic diketones: influence of dicarbonyl spacing on amine reactivity and toxicity. Chem Res Toxicol. 1988 Jul-Aug;1(4):200-3. IF.3.491
-    Rawlings CE 3rd, Giangaspero F, Burger PC, Bullard DE. Ependymomas: a clinicopathologic study. Surg Neurol. 1988 Apr;29(4):271-81. IF.1.556
-    Giangaspero F, Chieco P, Lisignoli G, Burger PC. Comparison of cytologic composition with microfluorometric DNA analysis of the glioblastoma multiforme and anaplastic astrocytoma. Cancer. 1987 Jul 1;60(1):59-65. IF.5.238
-    Giangaspero F, Doglioni C, Rivano MT, Pileri S, Gerdes J, Stein H. Growth fraction in human brain tumors defined by the monoclonal antibody Ki-67. Acta Neuropathol (Berl). 1987;74(2):179-82. IF.5.310
-    Giangaspero F, Doglioni C, Cattani MG, Fratamico FC.[Oncocytic variant of papilloma of the choroid plexus. Description of 2 cases] Pathologica. 1986 Jul-Aug;78(1056):511-6. Italian. No abstract available.
-    Allegranza A, Giangaspero F. [Demyelinating disease. Morphological and etiopathogenetic up-date] Pathologica. 1986 Mar-Apr;78(1054):143-64.
-    Giangaspero F, Salvi F, Ceccarelli C, Ambrosetto G, Govoni E, Tassinari CA. Familial amyloid polyneuropathy: report of a family. Clin Neuropathol. 1985 May-Jun;4(3):105-10. IF.1.200
-    Giangaspero F, Burger PC, Budwit DA, Usellini L, Mancini AM. Regulatory peptides in neuronal neoplasms of the central nervous system.Clin Neuropathol. 1985 May-Jun;4(3):111-5. IF.1.200
-    Giangaspero F, Dondi C, Scarani P, Zanetti G, Marchesini G.  Degeneration of the corticospinal tract following portosystemic shunt associated with spinal cord infarction. Virchows Arch A Pathol Anat Histopathol. 1985;406(4):475-81. IF.2.082
-    Giangaspero F, Burger PC, Osborne DR, Stein RB. Suprasellar papillary squamous epithelioma (“papillary craniopharyngioma”). Am J Surg Pathol. 1984 Jan;8(1):57-64. IF.4.535
-    Giangaspero F, Stracca V, Visona A, Eusebi V. Small-cell osteosarcoma of the mandible. Case report. Appl Pathol. 1984;2(1):28-31.
-    Pelusi G, Bondi A, Morichelli MP, Cavallina R, Giangaspero F, Eusebi V. Morpho-functional changes in normal cyclic endometrium. Basic Appl Histochem. 1984;28(2):151-8.
-    Giangaspero F, Manetto V, Ferracini R, Piazza G.  Squamous cell carcinoma of the brain with sarcoma-like stroma. Virchows Arch A Pathol Anat Histopathol. 1984;402(4):459-64. IF.2.082
-    Giangaspero F, Muhlbaier LH, Burger PC.The glioblastoma multiforme and the metastatic carcinoma: a morphometric study of nuclear size and shape. Appl Pathol. 1984;2(3):160-7.
-    Giangaspero F, Burger PC. Correlations between cytologic composition and biologic behavior in the glioblastoma multiforme. A postmortem study of 50 cases. Cancer. 1983 Dec 15;52(12):2320-33. IF.5.238
-    Anthony DC, Giangaspero F, Graham DG.  The spatio-temporal pattern of the axonopathy associated with the neurotoxicity of 3,4-dimethyl-2,5-hexanedione in the rat.J Neuropathol Exp Neurol. 1983 Sep;42(5):548-60. IF. 5.140
-    Burger PC, Dubois PJ, Schold SC Jr, Smith KR Jr, Odom GL, Crafts DC, Giangaspero F. Computerized tomographic and pathologic studies of the untreated, quiescent, and recurrent glioblastoma multiforme. J Neurosurg. 1983 Feb;58(2):159-69. IF.2.124
-    Zanetti G, Giangaspero F. Rhabdomyoblastic nature of cytoplasmic inclusions in malignant rhabdoid tumor. Hum Pathol. 1982 Apr;13(4):410. IF.3.322
-    Giangaspero F, Zanetti G, Mancini A, Rosito P. Sarcomatous variant of Wilms’ tumor. A light and immunohistochemical study of four cases. Tumori. 1981 Aug;67(4):367-73. IF.0.791
-    Giangaspero F, Vecchi V, Camerlo F, Pession A, Bondi A. [Childhood lymphoblast lymphoma (author's transl)] Haematologica. 1981 Feb;66(1):68-79.
-    Mancini AM, Giangaspero F, Tison V, Bertoni F.  Cytoplasmic hyaline in the lung. Hum Pathol. 1981 Feb;12(2):196-7.
-    Giangaspero F, Giardino R, Cola B, Tonielli E, Eusebi V. [Fuso-cellular carcinoma of the esophagus with aspects of microcytoma (oat cell)] Minerva Chir. 1980 Jan 15-31;35(1-2):77-83.
-    Eusebi V, Giangaspero F, Bondi A.  [Extra-lymphnodal pseudolymphoma] Recenti Prog Med. 1979 Aug;67(2):267-87.
-    Eusebi V, Betts CM, Giangaspero F.  Primary oat-cell carcinoma of the larynx. Virchows Arch A Pathol Anat Histol. 1978 Dec 4;380(4):349-54. IF.2.082
-    Eusebi V, Giangaspero F, Bondi A, Alampi G, Mancini A.  [Congenital benign reticulohistiocytoma type Hashimoto-Pritzker (author's transl)] Pathologica. 1978 Nov-Dec;70(1009-1010):693-7
-    Giangaspero F, Baruzzi G, Lemma E, Lorenzini P, Minghetti G, Ferracini R. [Spindle-cell carcinoma of the larynx: a clinico-pathological study of 5 cases (author's transl)] Pathologica. 1978 Sep-Oct;70(1007-1008):537-45